Phenotypic and genotypic detection of Basidobolus ranrum and Epidermophyton floccosum isolated from dust particles

Abstract

This study included isolation of Basidobolus ranrum and Epidermophyton floccosum from dust particles by diluted dust with distil water and culture on sabroude dextrose agar and potato dextrose agar, the two fungi diagnosed according to the shape and  color of colonies on plates, also the shape of spores and hypha under microscope.    DNA was extracted and amplified, then it was migrated on an agarose gel using the gene 18SrRNA. The results showed The two samples contained  bands for Basidobolus ranarum and Epidermophyton floccosum The sequencing technique was used for the diagnosed isolates after the completion of the PCR examination and the results of the examination were sent to Macrogen Company in South Korea to sequence the nitrogenous bases and  compared with the fungi sequences recorded in the NCBI Genebank website using MEGA10 program to draw the genetic tree of the fungus Basidobolus ranarum and Epidermophyton floccosum The results of the genetic tree analysis showed  a similarity between the fungi   with the species recorded on the Gene bank website,  And the fungus Basidobolus ranarum   was the closest similarity to the African isolate MW127174.1 Basidiobolus sp. South Africa, the fungus Epidermophyton floccosum   was the closest similarity to the Chinese isolate EF631604.1 Epidermophyton floccosum China .  Also the virulence factors of the two fungi tested and the results show that the proteinase, phospholipase and lipase enzymes were secreted from both fungi.