An Invitro Comparative Study on the Antimicrobial Efficacy of Nymphoidesindica and Nymphoidespeltata Extracts on Streptococcus Mutans, Lactobacillus and Actinomyces Viscosus.
Abstract
Dental caries is one of the most emerging problems in the oral cavity which affects most of the human population from the young age groups to adults. This disease inturn is caused by various microorganisms from the initiation to the progression. There are numerous preventive methods which can be tried followed to prevent the disease at its earliest onset.
AIM:Compared invitro the antimicrobial action of two aquaticplantsNymphoidesindicaandNymphoidesnpeltata on three different microorganisms thatcause dental caries (Streptococcus mutans, Lactobacillus and Actinomycesviscosus).
OBJECTIVE:Investigatedwhether the extracts of aquatic plants (Nymphoidesindica and Nymphoidespeltata) can be used as phytochemical oral rinse.
MATERIALS AND METHODOLOGY:
Crude sample NymphoidesindicaandNymphoidespeltataextract were prepared by Soxhlet extraction method 5. About 20gm of powdered sample material was uniformly packed into a thimble and extracted with 250ml of solvents separately. The process of extraction had to be continued for 24hrs (till the solvent in siphon tube of extractor become colourless). Then the extract were taken in a beaker, kept on a hot plate and heated at 30-40°C till all the solvent evaporated. The dried extract was then kept in refrigerator at 4°C for further use.Fractions and isolated compounds was evaluated for antimicrobialactivity in an integrated screening panel against the bacteria Streptococcusmutans, LactobacillusandActinomyces viscous. TheDisc Diffusion method was performed to test the antimicrobial efficacy.
RESULTS:
The values obtained for zone of inhibition in term of millimetre was tabulated and the mean values were obtained. Statistical analysis was done for evaluation, interpretation and presentation of the data obtained. The values were subjected to statistical analysis using paired ‘t’ test and mean ± standard deviation. Results obtained from the study showed Nymphoidesindicaextract exhibited maximum activity against Streptococcus mutans (24.33±4.51), followed by Nymphoidesindica extract against Lactobacillus acidophilus (22.33±3.06), and followed by Actinomyces.viscous (19.67±2.52) respectively at the concentration of 60µlcompared to Nymphoidespeltata extract with respect to commercially available oral rinse (0.2% Chlorhexidinegluconate).