Biosynthesis of Nano Zinc from Honey Solution and their Use in Controlling the Fungus Bipolaria Oryzae that Causes Brown Spot Disease on Rice

Abstract

This study included the biosynthesis and characterization of nano ZnONPs using honey and an evaluation of their effectiveness in inhibiting the growth of the fungus Bipolaria oryzae that causes brown spot disease on rice. The ability of the honey solution to produce nano zinc was tested by mixing 10 ml of honey solution with 100 ml of zinc acetate solution at a concentration of 1 mm dissolved in deionized distilled water and gradually heated to 80 °C for 20 minutes, observing the color change that started at 60 °C. nano Zinc has been characterized using several techniques. The results of UV-spectrophotometer analysis, which showed a strong absorption peak at 390nm, X-ray diffraction (XRD) results revealed the crystalline nature and the average size of nano zinc was 17.64nm. The results of the scanning electron microscope (SEM) showed that nano zinc appear in a homogeneous spherical shape in size. In contrast, the correctness of the surface shape of the nanoparticles of bio zinc oxide made from honey was confirmed by taking a two- and three-dimensional image using the atomic force microscope The Atomic Force microscopes (AFM), also used Fourier-transform infrared spectroscopy (FTIR), which showed that the effective group related to zinc oxide was in the range of 483.71.The results also showed that nano zinc  were highly effective in inhibiting the growth of the fungus Bipolaria oryzae that causes brown spot disease on rice, where the percentage of inhibition was 100% when using the concentration of 20 mg/ml.