Antibiotic Susceptibility Comparison of Environmental and Clinical Isolates of Staphylococcus Aureus

Abstract

Objectives: This study aimed to investigate the differences between clinical and natural Staphylococcus aureus. Isolated these same types environmentally and compare their resistance to antibiotics.

Methods: During the period from October 2021 to March 2022, 20 environmental samples and 20 samples from patients were collected. The bacterial diagnosis included colony culture, gram stain, and biochemical test, and these isolates were confirmed using VITEK®2 Compact. The research additionally included a study of sensitivity to specific antibiotics. Kirby-Bauer method is used for testing antimicrobial Susceptibility and reading the result depending on CLSI 2021.

Results: The sensitivity of these bacteria to antibiotics was studied showed that staphylococcus was 100% sensitive to Erythromycin 10ug, and Cefotaxime 30ug and Chloramphenicol 10ug to both clinical and environmental isolates, while Tetracycline 10ug Levofloxacin 15ug Ciprofloxacin 10ug, Gentamycin 10ug, and Azithromycin 10ug are sensitive only to clinical isolate, further Cefixime 5ug were sensitive only for environmental isolates and it showing sensitivity to clinical isolates, from the previous we note that notice the environmental isolates for staphylococcus aureus in the study area was higher resistance than clinical.

Conclusion: This study discovered that multidrug-resistant of environmental S.aureus isolates in natural habitats such as water sources could be identified as more dangerous than in clinical habitats, So our conclusion indicates that this is a public health issue that demands immediate attention from public health officials to treat it properly and as quickly as possible.