Determination of human sex in forensic cases by the possibility of mono- and duplex -polymerase chain reaction.

Abstract

Gender validation is seen as an important aspect of data validation, for demographic information, estimating age and size by determining sex can help to lessen the risk of forensic cases being matched based on gender. Therefore, the use of sexual genes (SRY, AMEL) is essential in determining the sex in forensic issues. The work was done on fifteen samples, 7 of them were males and 8 were females. Through the polymerase chain reaction, the SRY and AMELY genes indicated that they are present on the Y chromosome only, as both males were amplified without females, and this was also proven by the human gene hTERT 98 bp Where an internal control was used with the SRY gene in duplex PCR. These results in these genes indicated specificity these genes for sex determination