LC/MS Characterization of Flavonoids Glycosides from Leaves Ethanolic Extract of Bougainvillea Spectabilis Cultivated in Iraq

Abstract

Objective: The goal of this study was to detect the major compounds found in the leaves ethanolic extract of Iraqi cultivated Bougainvillea spectabilis. Methods: Plant materials were collected from Baghdad, Iraq, deffated with n-hexane, and extracted with ethanol. Then, the leaves ethanolic extract was analyzed by Liquid Gas Chromatography/Mass Spectroscopy (LC/Mass). Results: Flavonoid glycosides (Kaempferol-3-O-glucoside, Kaempferol-3-O-rutinoside, 3-Rha-7-Rha Quercetin, 3-O-Neohesperidoside Quercetin) were the major compounds obtained from the LC/Mass analyses.  The structure was characterized using standard retention times, [M-H] data, and MS/MS fragmentation peaks. Conclusion: This study found that Iraqi cultivated Bougainvillea spectabilis differs from those grown in other soils in that it lacks free flavonoids and instead contains sugar conjugated flavonoids linked via glycosidic linkage.